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11.
M. Riedy H. Matoba N. K. Vøllestad C. R. Oakley S. Blank L. Hermansen P. D. Gollnick 《Histochemistry and cell biology》1984,80(6):553-557
Summary Biopsy samples from the vastus lateralis muscle (VLM) of man were examined for fiber composition at rest and at selected intervals
during prolonged exercise ranging in intensity from 40% to 75% of the total body maximal oxygen uptake (VO
2max) and one-min bouts of exercise at 150%VO
2max. Because of the heterogeneity of fibers in human VLM, studies were also completed where the effect of exercise on the fiber
composition of the rat soleus muscle (SM) was examined. In some animals the SM from one hindlimb was removed 9 days prior
to their being exercised after which the remaining SM was removed. Exercise reduced muscle glycogen in all experiments. In
the studies with man, blood lactate exceeded 17 mmoles/l after the heavy exercise but was largely unchanged by endurance exercise.
Colonic temperature of the exercised rats exceeded 40° C. In studies where fibers were identified only as type I and type
II, type II fibers in the VLM of all samples (16) taken at rest averaged 61.2±12.5% as compared to 59.0±12.0% after exercise
(54 biopsy samples). In a second series of studies with man where the subtypes of type II fibers were identified, there were
also no differences in fiber composition of the VLM after varying periods of exercise. Glycogen content and percent fiber
composition were the same in right and left SM obtained from rested rats. Exercise (30 to 40 min) did not alter the fiber
composition of the rat SM. These data demonstrate that the histochemically demonstratable myofibrillar actomyosin ATPase of
skeletal muscle is not altered by a single exercise bout. 相似文献
12.
The ciliate Mesodinium rubrum has excited considerable interest because it has been reported to contain cryptophycean endoxymbionts which lack nuclei (Taylor et al., 1969, 1971). Hibberd (1977), however, has recently shown that in Mesodinium from the United Kingdom the cryptophycean chloroplasts and mitochondria share a common cytoplasm with an apparent cryptophycean nucleus. This cytoplasm is separated from the ciliate cytoplasm by a single membrane. Here we confirm, through extensive observation including serial sectioning, the presence of multiple, discrete, anucleate chloroplast-mitochondrial complexes in a population of Mesodinium from British Columbia. In addition to the normal ciliate macro- and micronuclei, however, these cells contain a third type of nucleus, possibly of cryptophycean origin, which is apparently in its own membrane-bound cytoplasm. This suggests the possibility of a new type of endosymbiotic organization in which endosymbiont organelles have become separated into discrete cytoplasmic compartments. 相似文献
13.
Burks Oakley II Sheldon S. Miller Roy H. Steinberg 《The Journal of membrane biology》1978,44(3-4):281-307
Summary We have studied the hyperpolarizing, electrogenic pump located on the apical membrane of the retinal pigment epithelium (RPE) in anin vitro preparation of bullfrog RPE-choroid. Changes in RPE [K+]
i
alter the current produced by this pump. Increasing [K+]
o
in the solution perfusing thebasal membrane increases RPE [K+]
i
(measured with a K+-specific microelectrode), and also depolarizes theapical membrane. This depolarization is due to a decrease in electrogenic pump current flowing across the apical membrane resistance, since it is abolished when the pump is inhibited by apical ouabain, by cooling the tissue, or by 0mm [K+]
o
outside the apical membrane. Removal of Cl– from the solution perfusing the basal membrane abolishes the K+-evoked apical depolarization by preventing the entry of K+ (as KCl) into the cell. We conclude that the increase in [K+]
i
causes the decrease in pump current. This result is consistent with the finding that [K+]
i
is a competitive inhibitor of the Na+–K+ pump in red blood cells.It is possible that the light-evoked changes in [K+]
o
in the distal retina could alter RPE [K+]
i
, and thus could affect the pump from both sides of the apical membrane. Any change in pump current is likely to influence retinal function, since this pump helps to determine the composition of the photoreceptor extracellular space. 相似文献
14.
15.
The structure of the dinoflagellate chromosome is examined using spread whole mounts and serial sectioning. Evidence is presented that the chromosome is composed of a toroidal bundle of DNA strands which is coiled plectonemically into a tight double helix. This structure is apparent in interphase whole-mount chromosomes and by careful analysis of serial sectioning can be shown in all mitotic stages. It is particularly apparent in the arms of replicating Y shaped chromosomes which often uncoil slightly (either naturally or as a result of the fixation used). A model is presented which is based on these results and recent genetic and biochemical data. 相似文献
16.
Threatened abortion, hormone therapy and malformed embryos 总被引:1,自引:0,他引:1
G P Oakley 《Teratology》1979,20(3):481-482
Causal relations between maternal genital bleeding, supportive hormone therapy and external malformations of the embryos were investigated with special reference to the critical period of organogenesis. This was done using morphological and obstetrical data obtained by Nishimura and his associates from 667 undamaged embryos derived from induced abortions whose mothers had genital bleeding in early pregnancy. In addition, data from 90 embryos with polydactyly and 38 with limb reductions in the Nishimura collection were used for case history studies. Evidence was presented to demonstrate that, for major malformations such as CNS anomalies, cleft lip, polydactyly and limb reductions, maternal genital bleeding was not a cause but a consequence of the conception of an abnormal embryo. No indication was revealed that exogenous female hormones currently used in Japan for preventing miscarraiges could produce major malformations recognizable at the embryonic stage, including limb reductions, nor salvage the severely malformed embryos. This does not however mean to exclude the possible relationship of progestogens/estrogens intake during early pregnancy with an increased incidence at birth of certain internal and/or external malformations. It was suggested that most, if not all, of the minor anomalies observed at certain embryonic stages are kinds of normal variants without any functional impairment of embryonic development. 相似文献
17.
18.
19.
Rats were used in this study to determine the time course of conversion of muscle fiber types. The right or left gastrocnemius muscle was removed thereby causing an overload on the ipsilateral soleus and plantaris muscles. The contralateral limb served as a control. The type II to type I fiber conversion was followed histochemically in the soleus and plantaris muscles for one to six weeks following surgery. Muscle sections were stained for myofibrillar actomyosin ATPase and NADH tetrazolium reductase. The type I population in the soleus muscle was 99.3% six weeks after synergist removal. The plantaris muscle underwent a two fold increase in the percentage of type I fibers after six weeks. Transitional fibers were prominent in the plantaris muscle and reached their peak at 4% (P less than 0.05) of the total population, four weeks after surgery. 相似文献
20.